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aureus biofilms were formed with or without 2 mg/mL nicotine in 96-well polystyrene microplates, washed gently with PBS, and then stained with 200 L of 5 g/mL wheat germ agglutinin (WGA)-Alexa Fluor 350 fluorescent conjugate (ThermoFisher, USA)

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Then, four girls high schools were selected from each region as a cluster ( n = 16 schools)

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