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The indicator cells were cultivated overnight with methionine (in the sulfur auxotroph) and GSH (in the gsh1 strain) in the pre-cultures and starved without these sources for 5-6 hours before re-inoculating at an initial OD 600 nm of 0.15 in media containing 100 M of GSH (for the sulfur auxotrophic strain) and no GSH for the glutathione deficient strain

Polletta L, Vernucci E, Carnevale I, Arcangeli T, Rotili D, Palmerio S, Steegborn C, Nowak T, Schutkowski M, Pellegrini L, Sansone L, Villanova L, Runci A, Pucci B, Morgante E, Fini M, Mai A, Russo MA, Tafani M

Sulforaphan ist zudem in der Lage, die reaktiven Sauerstoffverbindungen, die durch UV-Strahlen ausgelst werden, zu neutralisieren und das Melanozyten Stimulierende Hormon -MSH zu hemmen

Disclosure statement The authors declare that they have no conflict of interest