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Methionine SAM (via MAT) methyl donor formation Homocysteine remethylation methionine (requires B12 ) Transsulfuration (CBS & CGL, needs B6 ) cysteine Cysteine + glutamate + glycine GSH (glutathione synthetase) Nutrient supports: B12, B6, folate for methylation & transsulfuration Sulfur-rich foods (garlic, onion, broccoli) Protein sources (eggs, fish, legumes) for amino acids Glutathione synthesis is the step-by-step process by which the body creates its master antioxidant to support detoxification, immunity, and cellular health

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In our study, MTX was shown to significantly elevate MDA levels while reducing GSH levels in liver tissues, contributing to oxidative stress and the formation of lipid peroxides as observed in other studies

This is non-negotiable for reproducible research