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In the United States, tobacco use continues to be a leading cause of preventable disease, disability and death

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26 THE NINETEENTH CENTURY After the isolation of nicotine from tobacco leaves in 1828, 4 the medical world became yet more mistrustful of tobacco as a general treatment, now aware that the plant contained a dangerous alkaloid

Materials and methods Tobacco leaves and chemicals Rotten tobacco leaves (Yunyan 87) were collected from Changsha, Changde and Liuyang, Hunan Province, China, in 2022

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