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Purifications were conducted by preparative RP-HPLC, eluting with a linear gradient (20 ml min 1 ) and using a binary solvent system consisting of H 2 O:MeCN:TFA (buffer A, 95:5:0.1

Tirzepatide: A novel dual glucose-dependent insulinotropic polypeptide and glucagon-like peptide-1 receptor agonist for the treatment of type 2 diabetes

Let the pen warm up at room temperature for about half an hour before injecting

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One common mistake is stopping during the worst of the adjustment period, usually around days five through seven, and then restarting a week later